TABLE 4

Average parameter estimates (n = 100 repeats) for time-dependent inhibition with non-Michaelis-Menten (enzyme inhibitor inhibitor) biphasic (BP) kinetics

Data were simulated with the following parameters: BP: K_I1 = 10 µM, K_I2 = 100 µM, k_inact1 = 0.0025 min⁻¹, k_inact2 = 0.025 min⁻¹, k_inact2/K_I2 = 2.5 × 10⁻⁴ min⁻¹ µM⁻¹.

Model (% Error in Simulated Data)	Parameter	Numerical Method^{^a}	Modified (BP) Replot Method^{^a}	Standard Replot Method^{^a}^,^{^b}
BP (10%)	K_I1	8.9–10.9	0.1–25.6	2.9–67.4
	k_inact1	5 × 10⁻⁷–0.07	0.0017–0.017	NE
	k_inact2	0.012–0.032	NE	0.007–0.03
	k_inact2/K_I2 (×10⁻⁴)	1.3–3.2	0.04–16	NE
BP (5%)	K_I1	9.4–10.6	0.09–26.1	16.8–148.1
	k_inact1	2 × 10⁻⁶–0.06	0.0007–0.008	NE
	k_inact2	0.021–0.029	NE	0.013–0.038
	k_inact2/K_I2 (×10⁻⁴)	2.1–2.9	0.4–9.8	NE
BP (2.5%)	K_I1	9.7–10.3	0.2–30.1	44.1–158.9
	k_inact1	0.0014–0.0038	0.0007–0.005	NE
	k_inact2	0.023–0.027	NE	0.019–0.035
	k_inact2/K_I2 (×10⁻⁴)	2.3–2.7	2.3–2.8	NE

NE, not estimated.
↵^a Data are represented as a range of ±1 S.D. determined from the log normal distribution of 100 runs.
↵^b Parameters for the standard replot method are K_I and k_inact.