TABLE 1

Methods for immunoblot analysis

ProteinPrimary AntibodyPrimary Antibody SourceCell FractionPrimary Antibody ConcentrationPrimary Antibody Blocking Buffer DilutionSecondary AntibodySecondary Antibody SourceSecondary Antibody ConcentrationSecondary Antibody Blocking Buffer DilutionProtein Molecular Mass
kDa
Nrf28882SCell SignalingaNuclear1:10005% BSAAntirabbitSigmab1:10002% NFDM∼110
Nqo1ab2346AbcamcCytosolic1:10002% NFDMAntigoatSigmab1:10,0002% NFDM∼32
Ho-1SPA-895Stressgen BioreagentsdCytosolic1:20001% NFDMAntirabbitSigmab1:20002% NFDM∼33
GclcWashingtoneCytosolic1:20,0002% NFDMAntirabbitSigmab1:20,0002% NFDM∼75
GclmWashingtoneCytosolic1:20,0002% NFDMAntirabbitSigmab1:20,0002% NFDM∼28
Mrp3SC-5775Santa CruzfMembrane1:20002% NFDMAntiratSigmab1:20002% NFDM∼180
Mrp4ab15602AbcamcMembrane1:20002% NFDMAntiratSigmab1:20002% NFDM∼160
TBPab51841AbcamcNuclear1:10002% NFDMAntimouseSigmab1:30002% NFDM∼38
β-actinab8227AbcamcCytosolic1:35002% NFDMAntirabbitSigmab1:20002% NFDM∼42
  • BSA, bovine serum albumin; NFDM, nonfat dry milk.

  • a Cell Signaling Technology (Danvers, MA).

  • b Sigma-Aldrich (St. Louis, MO).

  • c Abcam (Cambridge, MA).

  • d Stressgen Bioreagents (Ann Arbor, MI).

  • e University of Washington (Seattle, WA), Terry Kavanagh.

  • f Santa Cruz Biotechnologies (Santa Cruz, CA).