Protein | Primary Antibody | Primary Antibody Source | Cell Fraction | Primary Antibody Concentration | Primary Antibody Blocking Buffer Dilution | Secondary Antibody | Secondary Antibody Source | Secondary Antibody Concentration | Secondary Antibody Blocking Buffer Dilution | Protein Molecular Mass |
---|---|---|---|---|---|---|---|---|---|---|
kDa | ||||||||||
Nrf2 | 8882S | Cell Signalinga | Nuclear | 1:1000 | 5% BSA | Antirabbit | Sigmab | 1:1000 | 2% NFDM | ∼110 |
Nqo1 | ab2346 | Abcamc | Cytosolic | 1:1000 | 2% NFDM | Antigoat | Sigmab | 1:10,000 | 2% NFDM | ∼32 |
Ho-1 | SPA-895 | Stressgen Bioreagentsd | Cytosolic | 1:2000 | 1% NFDM | Antirabbit | Sigmab | 1:2000 | 2% NFDM | ∼33 |
Gclc | – | Washingtone | Cytosolic | 1:20,000 | 2% NFDM | Antirabbit | Sigmab | 1:20,000 | 2% NFDM | ∼75 |
Gclm | – | Washingtone | Cytosolic | 1:20,000 | 2% NFDM | Antirabbit | Sigmab | 1:20,000 | 2% NFDM | ∼28 |
Mrp3 | SC-5775 | Santa Cruzf | Membrane | 1:2000 | 2% NFDM | Antirat | Sigmab | 1:2000 | 2% NFDM | ∼180 |
Mrp4 | ab15602 | Abcamc | Membrane | 1:2000 | 2% NFDM | Antirat | Sigmab | 1:2000 | 2% NFDM | ∼160 |
TBP | ab51841 | Abcamc | Nuclear | 1:1000 | 2% NFDM | Antimouse | Sigmab | 1:3000 | 2% NFDM | ∼38 |
β-actin | ab8227 | Abcamc | Cytosolic | 1:3500 | 2% NFDM | Antirabbit | Sigmab | 1:2000 | 2% NFDM | ∼42 |