TABLE 3

Histopathological analysis of livers from APAP-treated WT and Nrf2-null mice

Livers were removed from WT and Nrf2-null mice 4 and 24 hours following vehicle or APAP (200, 400, or 600 mg/kg) treatment and fixed in 10% formalin prior to paraffin embedding and staining with hematoxylin and eosin. Liver sections were evaluated for the severity of degenerative and necrotic changes in the centrilobular regions as previously described (Manautou et al., 1994; Aleksunes et al., 2005). Liver samples with grades greater than 2 are considered to have significant injury. Data were rank ordered prior to statistical analysis.

Histologic Grade
012345% > 2Total
WT
 4 hours
  Male
   Vehicle303
   400 mg APAP/kg50*5
  Female
   Vehicle303
   400 mg APAP/kg404
 24 hours
  Male
   Vehicle303
   400 mg APAP/kg1480*5
  Female
   Vehicle303
   400 mg APAP/kg505
Nrf2-null
 24 hours
  Male
   Vehicle404
   200 mg APAP/kg111260*5
  Female
   Vehicle606
   200 mg APAP/kg3104
   400 mg APAP/kg1211205
   600 mg APAP/kg12175*4
  • * Statistical difference (P < 0.05) from control mice of the same genotype and sex.