Internal standard | Acetaminophen-d4 | OH-bupropion-d6 | 5-OH-rosiglitazone-d4 | N/Aa | N/Aa | N/Aa | 1’-OH-midazolam-d4 |
MS mode | Positive | Positive | Positive | Positive | Positive | Positive | Positive |
Analyte m/z transition | 152.04 > 109.93 | 256.22 > 238.23 | 374.19 > 151.07 | 312.05 > 231.15 | 235.24 > 150.09 | 258.24 > 157.08 | 341.93 > 324.14 |
Internal standard m/z transition | 156.1 > 114.0 | 262.25 > 244.27 | 378.24 > 155.06 | N/Aa | N/Aa | N/Aa | 346.17 > 328.13 |
Capillary voltage (Kv) | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
Cone voltage (V) | 25 | 16 | 38 | 22 | 30 | 30 | 34 |
Collision energy (Ev) | 15 | 12 | 24 | 18 | 18 | 35 | 20 |
Desolvation temperature (°C) | 350 | 350 | 350 | 350 | 350 | 350 | 350 |
Source temperature (°C) | 120 | 120 | 120 | 120 | 120 | 120 | 120 |
Standard curve range (nM) | 20 ∼2000 | 5 ∼2000 | 2∼2000 | 100 ∼2000 | 50 ∼2000 | 2 ∼2000 | 5 ∼2000 |
Retention time (min) | 2.9 | 8.1 | 6.8 | 4.2 | 3.7 | 6.9 | 9.3 |
Mobile phase gradient: %B (min)b | 55 (0) → 100 (10) → 55 (10.1) → 100 (16) |