UTL-5g | DCA | ISOX | Zileuton (Internal Standard) | |
---|---|---|---|---|
MS mode | Positive | Positive | Positive | Positive |
m/z transition | 271.17 > 109.96 | 161.92 > 125.95 | 128.05 > 109.96 | 237.08 > 161.03 |
Capillary voltage (Kv) | 3 | 3 | 3 | 3 |
Cone voltage (V) | 20 | 35 | 15 | 16 |
Collision energy (Ev) | 16 | 18 | 10 | 12 |
Desolvation temperature (°C) | 350 | 350 | 350 | 350 |
Source temperature (°C) | 120 | 120 | 120 | 120 |
Retention time (min) | 16.86 | 14.63 | 11.85 | 13.53 |
Mobile phase gradient program: %B (min)a | 10 (0) → 95 (3.5) → 95 (19) → 10 (19.5) → 10 (24) |
↵a Mobile phase consisted of water (containing 0.1% formic acid) (A) and methanol (containing 0.1% formic acid) (B).