TABLE 2

The percentage of composition of 127I-ACTH (4–10)–derived materials in 127I-TIC in Fig. 3 from capillary UPLC-ICP-MS analysis of incubation of 10 μM 127I-ACTH (4–10) in human liver S9 at 37°C for 15 minutes in the absence and presence of 16.6× Halt PIC, 16.6× Halt PIC, 2 mM EDTA, 6 mM AEBSF, 300 μM bestatin or 6 mM AEBSF, and 300 μM bestatin

Metabolites% Composition
Liver S9S9 + PICS9 + PIC + EDTAS9 + AEBSFS9 + BestatinS9 + AEBSF + Bestatin
I-ACTH (4–10)10.256.892.827.327.436.7
M612.61.01.38.229.111.6
M59.337.23.822.27.912.0
M415.20.62.117.53.03.1
M30.80.9ND1.10.51.3
M251.2NDND21.95.52.4
M10.73.5ND1.826.732.9
  • ND, not detected; PIC, protease inhibitor cocktail.