TABLE 1

Between day precision and accuracy of calibration curve standards (n = 6) and four-level quality control (n = 18) standards for methamphetamine, 4-hydroxymethamphetamine, amphetamine, 4-hydroxyamphetamine, and norephedrine in monkey plasma

All CC and QC standard concentration values were presented in one decimal place except CC-8 and 9, and LQC and LLOQ which were presented in two decimal places.

Name	Calibration Curve Standard									Quality Control			LLOQ
Name	1	2	3	4	5	6	7	8	9	High	Middle	Low
Nominal concentration (ng/ml)	800	640	486.4	389.1	214	74.9	14.9	4.94	1.09	640	214	14.9	1.09
MA
Actual (ng/ml)	827.8	655.4	447.4	383.5	228.3	84.1	15.6	5.35	1.2	544.6	205.9	15.2	1
% Accuracy	103.5	102.4	92	98.6	106.4	112.2	104.7	108.3	110.1	85.1	96.2	101.5	91.9
% CV	7.8	10.1	11.7	14.8	4.3	18.1	9.6	9	9.6	13.7	11.5	7.6	23.1
4-OH MA
Actual (ng/ml)	869.7	605.4	497.4	383.3	228.2	83.9	15.5	5.34	1.23	578	214.4	15.3	1.1
% Accuracy	108.7	94.6	102.3	98.7	106.7	112.3	104.5	108.3	112.8	90.3	100.2	102.1	100.9
% CV	10.8	16.3	17.5	14.6	4.3	17.9	9.5	9.1	12.2	14.06	10.91	6.78	21.2
AM
Actual (ng/ml)	807.8	655.7	445.3	385.8	240.8	75.5	15.2	4.5	1.28	549.2	212.6	14.6	0.97
% Accuracy	101	102.5	91.6	99.2	112.5	100.8	102	91.1	117.4	85.8	99.3	97.5	89.4
% CV	7.1	8.7	10.2	12.8	8.1	10.4	10.6	19	8.6	13.5	10.4	10.4	27.2
4-OH AM
Actual (ng/ml)	779.4	671.7	465.3	439.8	245	78.7	16	4.7	1.29	560.6	232.7	15.4	1.06
% Accuracy	97.4	105	95.7	113	114.5	105.1	106.7	95.2	118.5	87.6	108.7	102.8	97.6
% CV	9	10.8	13	7.8	18.6	14.6	8.9	20.5	1.4	12.5	15.7	9.6	17
Norephedrine
Actual (ng/ml)	787.8	635.7	425.3	399.8	244.8	74.7	15.6	4.7	1.29	584.6	210.3	15.6	1.06
% Accuracy	98.5	99.3	87.4	102.7	114.4	99.7	104	95.2	118.5	91.3	98.3	104.1	97.6
% CV	7.6	9.2	11.2	7.2	7.6	9.4	12.7	16.2	6.6	15.4	10.2	6.2	19.5

CV (precision), coefficient of variation; HQC, high QC; LQC, lower QC; MQC, middle QC.