Nucleotide sequence for the primers used in quantitative real-time polymerase chain reaction
| mRNA | Left Primer (5′–3′) | Right Primer (5′–3′) | Predicted Product Size (Base Pair) |
|---|---|---|---|
| CBR1 | TCCACATTCAAGCAGAGGTG | CACTCTGCCTTGGGGTTTTA | 98 |
| CBR3 | ACCACATGGTAGAGTGGTGA | AGTGTGTCACATCGGAACCT | 99 |
| AKR1C3 | CCTGTGTGCAACCAGGTAGA | CCATAGGCAACCAGAACGAT | 95 |
| AKR1C14 | TTCCTGTACTGGGGTTTGGA | CAGAGTCAAAATGGCGGAAT | 108 |
| NQO1 | GCTTGACACTACGATCCGC | CACAGCAGCCTCCTTCATG | 117 |
| 11β-HSD1 | AGCATTGCCATCATCTCCTC | GTGGAAAAGAACCCATCCAG | 98 |
| CYP1A2 | GGCAGGTCAACCATGATGAG | CTTGTCGATGGCCGTGTT | 92 |
| CYP2D1 | CTGCAGGTGGACCTCAGTAA | ATGGGCTTCCAACCCTTC | 95 |
| CYP2E1 | GGGACATTCCTGTGTTCCAG | GTCTCGGAGAATGCTTAGGG | 104 |
| GAPDH | TGCCACTCAGAAGACTGTGG | GGATGCAGGGATGATGTTCT | 85 |
CBR1, carbonyl reductase type 1; CBR3, carbonyl reductase type 3; AKR1C3, aldo-keto reductase 1C3; AKR1C18, aldo-keto reductase 1C18; NQO1, NADPH quinone oxidoreductase type 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.