The kinetic parameters of probenecid acyl glucuronidation in HLHs in the absence or presence of 1 mM PMSF
Data are expressed as the mean ± S.D. of triplicate determinations.
| Group | Km | Vmax | CLint |
|---|---|---|---|
| μM | pmol/min per mg protein | nl/min per mg protein | |
| Control | 334.9 ± 0.8 | 21.3 ± 1.0 | 63.8 ± 3.0 |
| PMSF | 244.6 ± 37.9* | 25.9 ± 3.5 | 106.0 ± 3.5** |
↵* P < 0.01 and **P < 0.001 compared with control (0.5% DMSO).