TABLE 2

Inhibitory effect of several compounds on the uptake of [³H]nicotine or [³H]pyrilamine by rat hepatocytes

The uptake of [³H]nicotine (1 µCi/ml, 12 nM) and [³H]pyrilamine (1 µCi/ml, 50 nM) was measured at 37°C for 1 minute and 30 seconds, respectively, in the absence (control) or presence of compounds (1 mM). Each value represents the mean ± S.E.M. (n = 3–6).

Compounds	[³H]Nicotine	[³H]Pyrilamine
	% of control
Control	100 ± 2	100 ± 1
Nicotine	17.5 ± 0.5^**	9.34 ± 0.51^**
Pyrilamine	21.1 ± 0.8^**	4.60 ± 0.26^**
Propranolol	34.7 ± 0.9^**	2.46 ± 0.13^**
Clonidine	30.5 ± 1.0^**	12.4 ± 0.8^**
Amantadine	40.8 ± 1.1^**	15.9 ± 1.4^**
Verapamil	42.2 ± 1.0^**	5.29 ± 0.26^**
Quinidine	46.8 ± 1.3^**	3.74 ± 0.26^**
1-Methyl-4-phenylpyridinium	79.2 ± 2.8^**	73.2 ± 1.2^**
TEA	79.5 ± 5.7^**	72.8 ± 2.1^**
Choline	95.8 ± 3.1	78.8 ± 2.0^**
l-Carnitine	121 ± 6^**	80.3 ± 0.4^**
p-Aminohippurate	87.8 ± 3.0^**	79.4 ± 2.1^**

↵** P < 0.01, significantly different from control.