Suppression of paracetamol (CYP1A2), hydroxybupropion (CYP2B6), and 1′ hydroxymidazolam (CYP3A4) formation after treating HepaRG cells with increasing concentrations of LPS (1.37–333 ng/ml), IL-6 (0.123–30 ng/ml), and IL-18 (2.06–500 ng/ml)

Data were normalized to untreated cells and fit to a four-parameter dose-response model. Mean and standard deviation values are from three different plates. For L-18, no Emin is quoted owing to the lack of any dose-response.

EnzymeMetaboliteLPSIL6IL18 EC50
% of controlng/ml% of controlng/ml
CYP1A2Paracetamol47.6 ± 7.2>33335.2 ± 7.78.96>500
CYP2B6Hydroxybupropion47.4 ± 2.1811620.4 ± 1.280.970>500
CYP3A41′-Hydroxymidazolam39.1 ± 4.637.8541.1 ± 3.442.89>500