TABLE 1

Suppression of gene expression for CYP1A2, CYP2B6, and CYP3A4 after treating HepaRG cells with increasing concentrations of LPS (1.37–333 ng/ml), IL-6 (0.123–30 ng/ml), and IL-18 (2.06–500 ng/ml)

Data were normalized to untreated cells and fitted to a four-parameter dose-response model. Mean and standard deviation values are from three different plates. For IL-18, no Emin is quoted owing to the lack of any dose-response.

EnzymeLPSIL6IL18 EC50
EminEC50EminEC50
% of controlng/ml% of controlng/ml
CYP1A228.8 ± 20.037.97.83 ± 5.300.452>500
CYP2B631.3 ± 6.1219.84.64 ± 0.980.388>500
CYP3A44.39 ± 2.73<1.370.998 ± 0.418<0.123>500