TABLE 5

Phase II DMEs

Activities, protein, and mRNA relative levels. Results are relative mean ± S.D.; controls set to 100%; n = 3 for catalytic activities and immunoblotting; n = 4 for mRNA quantification. mRNA quantity expressed as a fold change.

	Catalytic Activities		Immunoblotting			mRNA Quantity
	Control	Obese		Control	Obese		Control	Obese
Enzyme			Class			Isoform
Liver
UGT1A	100.0 ± 1.1	167.8 ± 6.7^*		100.0 ± 8.7	232.7 ± 50.8^*		1.00 ± 0.41	2.71 ± 0.41^*
GST	100.0 ± 4.0	77.7 ± 4.2^*	GSTA	100.0 ± 13.4	127.9 ± 9.1^*	GSTA1/2^a	1.00 ± 0.10	2.32 ± 1.80
						GSTA3	1.00 ± 0.20	0.97 ± 0.30
						GSTA4	1.00 ± 0.29	1.03 ± 0.23
			GSTM	100.0 ± 10.4	127.7 ± 7.8^*	GSTM1	1.00 ± 0.30	1.47 ± 0.30
				100.0 ± 10.4	127.7 ± 7.8^*	GSTM3	1.00 ± 0.46	2.38 ± 0.63^*
			GSTP	100.0 ± 6.4	77.6 ± 13.4^*	GSTP1/2^a	1.00 ± 0.30	0.20 ± 0.10^*
Small Intestine
UGT1A	n.d.	n.d.		100.0 ± 26.3	118.2 ± 43.1		1.00 ± 0.47	1.02 ± 0.39
GST	100.0 ± 10.4	99.4 ± 13.0	GSTA	100.0 ± 5.0	162.8 ± 16.5^*	GSTA1/2^a	1.00 ± 0.22	0.75 ± 0.24
						GSTA3	1.00 ± 0.40	1.50 ± 0.33
						GSTA4	1.00 ± 0.45	0.84 ± 0.48
			GSTM	100.0 ± 10.6	125.1 ± 15.4	GSTM1	1.00 ± 0.25	0.86 ± 0.18
				100.0 ± 10.6	125.1 ± 15.4	GSTM3	1.00 ± 0.06	0.81 ± 0.11
			GSTP	100.0 ± 7.2	198.8 ± 65.4^*	GSTP1/2^a	1.00 ± 0.29	0.91 ± 0.12

n.d., Not detected.
↵^a Two isoforms analyzed together.
↵^* Statistically significant (P < 0.05).