TABLE 3

Antioxidant enzymes

Activities, protein, and mRNA relative levels. Results are mean ± S.D.; controls set to 100%; n = 3 for catalytic activities and immunoblotting; n = 4 for mRNA quantification. mRNA quantity expressed as a fold change.

	Catalytic Activities		Immunoblotting		mRNA Quantity
	Control	Obese	Control	Obese	Control	Obese
Liver
SOD1	100.0 ± 1.5	104.8 ± 1.4	100.0 ± 6.9	114.5 ± 11.7	1.00 ± 0.46	1.36 ± 0.33
CAT	100.0 ± 1.4	99.3 ± 37.7	100.0 ± 3.2	88.7 ± 5.0^*	1.00 ± 0.37	1.19 ± 0.20
GPx^a	100.0 ± 0.5	78.3 ± 1.7^*	n.d.	n.d.	1.00 ± 0.31	3.21 ± 1.15^*
GR	100.0 ± 7.3	103.4 ± 2.6	100.0 ± 16.9	118.3 ± 14.0	1.00 ± 0.43	1.31 ± 0.48
TR	100.0 ± 12.3	107.2 ± 16.5	100.0 ± 13.8	66.40 ± 5.0^*	1.00 ± 0.46	0.79 ± 0.23
Small Intestine
SOD1	100.0 ± 4.5	106.0 ± 4.7	100.0 ± 10.0	151.4 ± 10.7^*	1.00 ± 0.44	0.52 ± 0.08
CAT	100.0 ± 7.7	61.2 ± 1.7^*	100.0 ± 10.6	160.4 ± 8.1^*	1.00 ± 0.66	1.15 ± 0.42
GPx^{^a}	100.0 ± 3.8	119.0 ± 7.0^*	100.0 ± 9.1	89.0 ± 7.2	1.00 ± 0.17	0.30 ± 0.12^*
GR	100.0 ± 2.6	89.8 ± 1.5^*	100.0 ± 7.3	147.7 ± 9.0^*	1.00 ± 0.07	0.94 ± 0.25
TR	100.0 ± 2.0	86.9 ± 7.5^*	100.0 ± 18.1	102.7 ± 11.9	1.00 ± 0.13	0.63 ± 0.12^*

n.d., Not detected.
↵^a Relative specific activity measured for all isoforms, protein, and mRNA GPx2-specific isoform only.
↵^* Statistically significant (P < 0.05).