TABLE 5

Expression level of IL-1β, IL-6, TNFα, and IL1-Ra in wild-type and PXR-KO PCHs

PCHs isolated from wild-type (n = 6) or PXR-KO mice (n = 6) were pretreated for 24 hours with either vehicle (0.1% dimethylsulfoxide) or PCN (10 μM). The cultures were split into two experimental groups (n = 3) and then were treated with vehicle, PCN, LPS (10 μg/ml), or were cotreated with PCN and LPS together for an additional 12 hours. Total RNA was isolated, and RT-qPCR was performed using custom designed primer pairs (Supplemental Table 4). Data are expressed as fold induction ± S.D.

Hepatocyte Cultures	Vehicle	LPS	PCN + LPS	PCN
Wild Type
IL-1β	1.0 ± 0.1	1723.2 ± 21.7^{^a}	884.4 ± 96.2^{^a}^,^{^b}	96.11 ± 8.3^{^a}
IL-6	1.0 ± 0.1	379.9 ± 5.1^{^a}	154.2 ± 17.9^{^a}^,^{^b}	15.1 ± 2.5^{^a}
TNFα	1.0 ± 0.2	54.2 ± 8.7^{^a}	24.5 ± 3.1^{^a}^,^{^b}	8.2 ± 1.2^{^a}
IL1-Ra	1.0 ± 0.3	35.2 ± 4.2^{^a}	15.3 ± 1.2^{^a}^,^{^b}	5.4 ± 0.5^{^a}
PXR-KO
IL-1β	204.4 ± 24.1^{^a}	3351.2 ± 294.4^{^a}^,^{^c}	4094.8 ± 201.2^{^a}^,^{^c}	246.11 ± 38.3^{^a}
IL-6	25.9 ± 2.9^{^a}	176.2 ± 5.1^{^a}	199.22 ± 11.9^{^a}	25.1 ± 4.6^{^a}
TNFα	5.8 ± 0.8^{^a}	34.2 ± 18.6^{^a}	38.4 ± 25.3^{^a}	8.1 ± 4.2^{^a}
IL1-Ra	3.7 ± 0.6^{^a}	21.9 ± 4.2^{^a}	25.3 ± 3.7^{^a}	5.2 ± 0.5^{^a}

↵^a Statistically significant compared with the vehicle-treated wild-type experimental group (P ≤ 0.05).
↵^b For the wild-type [PCN + LPS] experimental group, indicates a statistical difference when compared with LPS-treatment alone.
↵^c For the PXR-KO experimental group, indicates that IL-1β expression was enhanced in both of the LPS alone and the [LPS + PCN] experimental groups when compared with IL-1β expression levels in wild-type hepatocyte cultures.