TABLE 3

Expression profiling of primary cultures of mouse hepatocytes cotreated with LPS and PCN when compared with LPS alone

PCHs from wild-type mice were pretreated with PCN or vehicle (0.1% dimethylsulfoxide) for 24 hours (n = 6). Cultures were divided into two experimental groups and were treated with LPS alone or were cotreated with PCN and LPS together for an additional 12 hours (n = 3). Total RNA was isolated, and RT-qPCR was performed. Data are expressed as fold suppression ± S.D. in the cotreated (PCN + LPS) group when compared with LPS alone (P ≤ 0.05).