TABLE 2

MPTP N-oxygenation catalyzed by marmoset and human FMO1 and FMO3

Marmoset and human recombinant FMOs (20 pmol/ml) were incubated with 200 µM MPTP at 37°C for 10 minutes in the presence of an NADPH-generating system. Formations of PTP, MPDP+, and MPP+ by FMO were below the detection limits (for MPDP+ and MPP+, <0.1 nmol/min per nmol FMO; for PTP, <0.01 nmol/min per nmol FMO).

FMOMPTP N-Oxygenation
nmol/min per nmol FMO
Marmoset
 FMO12.9
 FMO314.5
Human
 FMO14.2
 FMO378.0