TABLE 2

Effects of IL-1β and IL-6 on uptake and efflux transporters mRNA expression in Hep:KC (1:0 and 1:0.4) cell cultures

Transporters	Exposure to IL-1β (6.25–5000 pg/ml), 4 Days		Exposure to IL-6 (6.25–5000 pg/ml), 4 Days
Transporters	Effects^{^a}	KC-Dependent Effects?	Effects^{^a}	KC-Dependent Effects?
NTCP	↓^{^b}	Yes	↓	—
OAT2	↔	—	↔	—
OATP1B1	↓^{^b}	Yes	↔	—
OATP1B3	↓^b	Yes	↓	—
OATP2B1	↓	—	↔	—
OCT1	↓	—	↓	—
BCRP	ND	—	ND	—
BSEP	↓	Yes	↓	—
MDR1	↔	—	↔	—
MRP2	↔	—	↔	—
MRP3	↔	—	↔	—
MRP4	ND	—	ND	—

↵^a Effects were noted as noneffect (↔) or downregulated (↓) ≤50% of untreated control in a concentration-dependent manner. Samples with Ct value >32 were designated as nondetectable (ND).
↵^b Effects were observed only in Hep:KC 1:0.4 but not 1:0 cultures.