TABLE 1 Enzyme kinetic parameters of UGTs toward OTS167
Incubations were performed in triplicate with OTS167 concentrations ranging from 0.5 µM to 100 µM. Data are shown as means ± S.E.M. Data were fitted using the substrate inhibition model Y = Vmax × X/[Km + X × (1 + X/Ki)], where Y is the reaction rate, X is substrate concentration, Vmax is apparent maximal velocity, Km is substrate concentration at which half-maximum velocity is achieved (Michaelis constant), and Ki is the inhibition constant. CLint = Vmax/Km.