TABLE 1

Canine cbr1 D218 and cbr1 V218 enzyme kinetics constants

SubstrateD218V218
KmVmaxkcataVmax/KmKmVmaxkcataVmax/Km
μMnmol/min per milligrammin−1mg/min × 10−3μMnmol/min per milligrammin−1mg/min × 10−3
Daunorubicinb188 ± 1446446 ± 361521134.2527 ± 13615539 ± 262350929.4
Menadioneb104 ± 502034 ± 3076719.6118 ± 512041 ± 3346717.3

  • ↵a Calculated assuming a MW of 32,800 g/mol.

  • ↵b Values represent the mean ± S.D. from two experiments performed in triplicate with independent protein preparations.