TABLE 3
Kinetic parameters of diltiazem deacetylation in RLM and recombinant Ces2a
Enzyme Source
K
m
V
max
CL
int
µM
nmol/min/mg
µL/min/mg
RLM
359.4 ± 9.7
11.6 ± 0.4
32.2 ± 0.3
Recombinant Ces2a
116.8 ± 5.3
18.3 ± 0.5
156.8 ± 3.1