In vitro activity of recombinant CYP3A enzymes toward midazolam
| rCYP3A5 | rCYP3A7 | rCYP3A4 | Midazolam Metabolite Formation | 1ʹ-OH MDZ/4-OH MDZ Ratio | |
|---|---|---|---|---|---|
| 1ʹ-OH MDZ | 4-OH MDZ | ||||
| pmol | pmol/min/pmol P450 | ||||
| — | — | 2.5 | 4.20 | 1.04 | 4.05 |
| 2.5 | — | — | 10.16 | 1.15 | 8.85 |
| — | 2.5 | — | 0.72 | 0.70 | 1.03 |
| 2.25 | 0.25 | — | 6.86 | 0.91 | 7.52 |
| 1.875 | 0.625 | — | 6.62 | 1.00 | 6.65 |
| 1.25 | 1.25 | — | 4.20 | 0.85 | 4.96 |
| 0.625 | 1.875 | — | 2.38 | 0.74 | 3.22 |
| 0.25 | 2.25 | — | 1.20 | 0.66 | 1.82 |