Doxycycline loading using 3.0 M sulfuric acid–loaded 20 μM DPPC/cholesterol liposomes
| Time/Temperature/Input Concentration of Doxycyclinea | Milligrams Captured |
|---|---|
| h/°C per mg/ml | % |
| 1/55/33 | 0.401 (4.86) |
| 1/55/8.25 | 1.54 (74.69) |
| 24/22/33 | 1.21 (14.77) |
| 24 /22 /8.25 | 2.02 (97.77) |
| 48/ 22/33 | 3.199 (38.77) |
| 48/22 /8.25 | 1.44 (69.57) |
↵a Loading conditions were 1, 24, or 48 hours on the shaker at either 55 or 22°C. A doxycycline concentration of either 8.25 or 33 mg/ml was added to the liposome mixture.