The effect of intracerebral injection of 5,7-DHT on the mRNA expression level of P450 genes in rat liver
The results are expressed as a fold change in relation to 2 housekeeping genes: the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene and β-actin gene controls. All values are the mean fold change calculated by the comparative 2−∆∆Ct method for control (n = 7) and the 5,7-DHT (n = 6) group.
| Gene Name | Fold Change Relative to GAPDH Gene | Statistical Significance | Fold Change Relative to β-Actin Gene | Statistical Significance |
|---|---|---|---|---|
| CYP1A1 | 3.76 ↑* | P < 0.05 | 5.76 ↑* | P < 0.05 |
| CYP1A2 | 1.96 ↑* | P < 0.05 | 1.62 ↑* | P < 0.05 |
| CYP2C11 | 1.35 ↑ | ns | 1.68 ↑* | P < 0.05 |
| CYP3A1 | 2.71 ↑* | P < 0.05 | 1.69 ↑ | ns |
| CYP3A2 | 1.03 | ns | 1.05 | ns |
ns, not significant.
↵* Statistically significant changes.