TABLE 2

Qualitative comparison of the effect of different agents or experimental conditions on the formation of ROP from AZD9819

All incubations and controls are conducted at 37°C for 2 hours unless specified otherwise.

Agent or Condition	Concentration of Agent	Control	ROP (% of Control)
Agent or Condition	Concentration of Agent	Control	Rat Plasma	Dog Plasma
Amber vial	N/A	Clear vial	95
Completely dark	N/A	Ambient light		101
NaN₃	1 and 50 mM	w/o NaN3	94 and 84
NaN₃	10 mM			119
Allopurinol	100 µM	w/o Allopurinol	99	102
KCN	50 mM	w/o KCN	209
Room temperature	N/A	37°C	57	74
On ice	N/A	37°C	9
Preheated plasma at 56°C for 1 hour	N/A	Plasma w/o preheating	61
Denatured plasma	1:1 MeCN	Plasma	3
Catalase	1000 unit/mL	w/o catalase	82
Ascorbic acid	1 mM	w/o added ascorbic acid	76
Ascorbic acid	50 mM	w/o added ascorbic acid	25	35
GSH	5 mM	w/o added GSH	33
GSH	50 mM	w/o added GSH	2	16