TABLE 1

Kinetic parameters derived for hesperetin sulfonation by cell lysate preparation and expressed SULT1A3 enzyme

Data are represented by mean ± S.E.

Enzyme Source	Metabolite	V_max	K_m	K_si	CL_in	Fitted Model
		nmol/min per mg	μM	μM	μl/min per mg
Cell lysate	H-3′-S	0.66 ± 0.08	12.9 ± 2.41	58.1 ± 13.7	51.2 ± 6.13	SI
Cell lysate	H-7-S	0.29 ± 0.04	14.8 ± 2.87	49.1 ± 11.6	19.6 ± 3.56	SI
SULT1A3	H-3′-S	3.46 ± 0.45	18.5 ± 3.44	37.9 ± 8.17	187 ± 10.2	SI
SULT1A3	H-7-S	1.43 ± 0.15	18.4 ± 2.82	36.4 ± 6.42	77.6 ± 8.33	SI