TABLE 2

Summary of optimal reaction conditions and projected cost savings to generate milk thistle flavonolignan glucuronides

CompoundOptimized ConditionsTypical Conditionsa
Silybin ASilybin BIsosilybin AIsosilybin BN/A
Enzyme sourceBLMsS9BLMsBLMsHLMs
Enzyme concentration (mg/ml)0.250.250.250.251.0
UDPGA (mM)0.80.80.80.84
Time (h)88412N/A
Cost (U.S. dollars)b4803104604802400
  • N/A, not applicable.

  • a Walker et al. (2014).

  • b Based on a 25-mg scale reaction.