TABLE 1

IC₅₀ values of HIV PIs against PMAT and OCT1–3

Concentration-dependent inhibition experiments were conducted on PMAT and OCT1–3 with IDT307 or ASP+ assays. IC₅₀ values were determined by nonlinear regression fit as described in Materials and Methods. Shown are the mean ± S.D. values from three independent kinetic experiments.

HIV PI	IC₅₀ (µM)
HIV PI	PMAT	OCT1	OCT2	OCT3
Indinavir	NI	33.4 ± 6.8^{^a}	NI	NI
Amprenavir	51.5 ± 8.8^{^a}	40.4 ± 7.3^{^a}	NI	NI
Nelfinavir	44.6 ± 8.0^{^a}	60.5 ± 10.9^{^a}	NI	NI
Atazanavir	49.5 ± 7.5^{^a}	NI	NI	NI
Tipranavir	8.9 ± 2.8	38.0 ± 5.8^{^a}	NI	NI
Lopinavir	1.4 ± 0.2	174 ± 40.1^{^a}	NI	NI
Ritonavir	6.0 ± 0.5	11.2 ± 3.5	78.9 ± 19.7^{^a}	59.8 ± 9.3^{^a}
Saquinavir	7.0 ± 1.0	44.8 ± 12.0^{^a}	NI	NI

NI, no significant concentration-dependent inhibition.
↵^a Partial inhibition at the highest concentration tested (50 µM) due to limited drug solubility.