TABLE 2

Derived kinetic parameters for OLZ oxidative pathways by HLMs in the presence of BSA [2% (w/v)]

Data are the mean ± S.D. for microsomes from five individual livers.

PathwayKm or S50Vmax
µMpmol/min per mg
N-demethylation22 ± 870 ± 15
7-hydroxylation14 ± 333 ± 19
N-oxidationa228 ± 107250 ± 70
2-hydroxylationb638 ± 35228 ± 11

  • ↵a Experimental data for one liver (H12) were best described by the Hill equation (n = 1.5).

  • ↵b Kinetic constants from fitting with the Hill equation (n = 0.6–0.9).