Kinetic parameters of calycosin and CG glucuronidation obtained from RLMs and RIMs
| Compound | Kinetic Parameters | RLMs | RIMs |
|---|---|---|---|
| Calycosin (7-OH) | Km (μM) | 1.61 ± 0.69 | 1.19 ± 3.45 |
| Vmax (nmol/min/mg) | 0.49 ± 0.069 | 0.068 ± 0.078 | |
| CL (ml/min/mg) | 304.35 | 57.14 | |
| Calycosin (3′-OH) | Km (μM) | 2.77 ± 0.18 | 6.1 ± 0.38 |
| Vmax (nmol/min/mg) | 7.12 ± 0.08 | 18.03 ± 0.23 | |
| CL (ml/min/mg) | 2570.4 | 2955.74 | |
| CG | Km (μM) | 101.28 ± 6.86 | 13.28 ± 1.12 |
| Vmax (nmol/min/mg) | 6.84 ± 0.165 | 24.52 ± 0.57 | |
| CL (ml/min/mg) | 67.54 | 1846.39 |