TABLE 1

Derived kinetic constants for cotinine N-glucuronidation by recombinant UGT2B10 and human liver microsomes (±BSA, 1% w/v)

Kinetic Parameter	Enzyme Source
Kinetic Parameter	Recombinant UGT2B10	HLM	HLM + BSA
K_m (mM)	2.78 ± 0.34	3.34 ± 0.39	1.85 ± 0.07^b
V_max (pmol/min.mg)	43.7 ± 2.29	297 ± 14.5^a	329 ± 4.56^b
CL_int (µl/min.mg)	15.9 ± 2.48	89.7 ± 7.99^a	178 ± 4.41^b

Kinetic parameters expressed as mean ± S.D. of four to six replicates.
↵^a P < 0.05 compared with recombinant UGT2B10.
↵^b P < 0.05 compared with HLM without BSA.