Transporter abundance levels in the HEK293 cells (SLC transporters), inverted membrane vesicles (ABC transporters), and sandwich-cultured human hepatocytes (SCHHs)
Transporter expression was determined from abundance levels of surrogate peptides measured by targeted LC-MS/MS analysis (CV of the quantification methods <15%) (Balogh et al., 2012; Li et al., 2008b; Li et al., 2009; Qiu et al., 2013).
| Surrogate peptide expression levels (pmol/mg membrane protein) | ||||||
|---|---|---|---|---|---|---|
| Transporter | HEK293 Cells | Membrane Vesicles | SCHH UU113 | SCHH UU115 | SCHH UU112 | SCHH UU125 |
| NTCP (SLC10A1) | 19.7 | NA | 0.38 | 0.53 | 0.63 | 0.27 |
| OATP1B1 (SLCO1B1) | 12.1a | NA | 3.82 | 4.46 | 2.05 | 4.64 |
| OATP1B3 (SLCO1B3) | 4.71a | NA | 0.45 | 1.01 | 0.79 | 1.69 |
| OATP2B1 (SLCO2B1) | 57.3a | NA | 1.30 | 1.66 | 1.67 | 0.65 |
| BCRP (ABCG2) | NA | 260 | 0.22 | 0.39 | 0.33 | 0.11 |
| MDR1 (ABCB1) | NA | 280 | 0.95 | 1.19 | 0.83 | 0.95 |
| MRP3 (ABCC3) | NA | 40.0 | 0.97 | 0.96 | 1.60 | 1.64 |
NA, not applicable.
↵a Values reprinted with permission from Karlgren et al. (2012b).