Concentration of KAE609 and its metabolites in human semen after an oral dose of 300 mg [14C] KAE609
The semen samples were extracted and analyzed by LC-MS/MS for metabolic profiling. The HPLC separation of KAE609 and metabolites was performed using a Zorbax SB-C18 column, as described in the Materials and Methods.
| Metabolite/Subject | Concentration of KAE609 and Metabolites in Human Semena | |||||||
|---|---|---|---|---|---|---|---|---|
| 5101 | 5102 | 5103 | 5104 | 5105 | 5106 | Mean | SD | |
| ngEq⋅h/ml | ||||||||
| M16 | 634 | 287 | 807 | 433 | 231 | 518 | 485 | 216 |
| M18 | 283 | 203 | 228 | 208 | 162 | 263 | 224 | 44 |
| M20.8 | ND | ND | 548 | 198 | ND | 189 | 312 | 205 |
| M21.8 | 823 | 123 | 279 | 143 | ND | 173 | 308 | 294 |
| M23 | 4699 | 1640 | 4023 | 4050 | 653 | 2332 | 2900 | 1599 |
| M32 | ND | 75 | 269 | 97 | 219 | 304 | 193 | 102 |
| M48 | ND | 61 | 331 | 115 | 231 | 287 | 205 | 114 |
| KAE609 | 1149 | 382 | 1138 | 475 | 167 | 369 | 613 | 423 |
| Total | 7588 | 2772 | 7623 | 5717 | 1663 | 4435 | 4966 | 2470 |
ND, not detectable.
↵a Semen samples were collected from subjects 5103 and 5106 at 6 hours postdose, subjects 5101 and 5105 at 12 hours postdose, and subjects 5102 and 5104 at 24 hours postdose.