Signature peptides for human FMO1, FMO3, and FMO5
| Protein | Signature Peptidea | Peptide Sequenceb | Start-Endc | Average Mass MH+d | MRM (m/z) | CE | |
|---|---|---|---|---|---|---|---|
| Precursor Ion | Product Ion | ||||||
| Da | V | ||||||
| FMO1 | FMO1_pep1_L | FTEHVEEGR | 43–51 | 1104.2 | 552.5 | 589.3 (y5) | 20 |
| FMO1_pep1_H | FTEHVEEG(R) | 1114.2 | 557.5 | 599.3 (y5) | 20 | ||
| FMO1_pep2_L | VEDGQASLYK | 345–354 | 1110.2 | 555.5 | 881.5 (y8) | 15 | |
| FMO1_pep2_H | VEDGQASLY(K) | 1118.2 | 559.6 | 889.5 (y8) | 15 | ||
| FMO3 | FMO3_pep1_L | FSDHAEEGR | 43–51 | 1048.1 | 524.5 | 561.3 (y5) | 20 |
| FMO3_pep1_H | FSDHAEEG(R) | 1058.1 | 529.5 | 571.3 (y5) | 20 | ||
| FMO3_pep4_L | SNDIGGLWK | 34–42 | 990.1 | 495.4 | 560.3 (y5) | 15 | |
| FMO3_pep4_H | SNDIGGLW(K) | 998.1 | 499.4 | 568.3 (y5) | 15 | ||
| FMO5 | FMO5_pep1_L | FQENPEEGR | 44–52 | 1106.1 | 553.4 | 587.3 (y5) | 18 |
| FMO5_pep1_H | FQENPEEG(R) | 1116.1 | 558.4 | 597.3 (y5) | 18 | ||
| FMO5_pep6_L | WATQVFK | 388–394 | 880.0 | 440.5 | 622.4 (y5) | 10 | |
| FMO5_pep6_H | WATQVF(K) | 888.0 | 444.5 | 630.4 (y5) | 10 | ||
↵a L and H indicate unlabeled and stable isotope-labeled peptides, respectively.
↵b Stable isotope-labeled amino acid residues are included in parentheses.
↵c Start and end residue positions of peptides in the corresponding full-length protein.
↵d Theoretical average mass of mono-protonated molecular ion.