Significance (P value) of differential expression in Fig. 7 determined by one-way analysis of variance and Tukey’s post-hoc analysis
| Gene Symbol | Uninfected versus | Hnf1 + Hnf4 versus | Hnf1 +Hnf4 +Gata4 versus | Hnf1a Hnf4a Fox2/3 versus | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Hnf1a | Hnf1a | Hnf1a | Hnf1a | Hnf1a | Hnf1a | Hnf1a | Hnf1a | Hnf1a | Hnf1a | |
| Hnf4a | Hnf4a | Hnf4a | Hnf4a | Hnf4a | Hnf4a | Hnf4a | Hnf4a | Hnf4a | Hnf4a | |
| Gata4 | Foxa2/3 | Gata4 | Gata4 | Foxa2/3 | Gata4 | Foxa2/3 | Gata4 | Gata4 | ||
| Foxa2/3 | Foxa2/3 | Foxa2/3 | Foxa2/3 | |||||||
| Proximal tubule markers | ||||||||||
| Slc47a1 | *** | – | *** | * | *** | – | *** | *** | – | ** |
| Ggt1 | *** | * | *** | ** | *** | *** | *** | ** | – | * |
| Slc22a6 | *** | – | – | – | *** | *** | *** | – | – | – |
| Slc34a1 | *** | – | – | – | *** | *** | *** | – | – | – |
| Hepatocyte markers | ||||||||||
| Fabp1 | – | * | ** | *** | * | ** | *** | – | *** | *** |
| Trf | – | – | *** | *** | – | *** | *** | *** | *** | *** |
| Ttr | – | – | *** | * | – | *** | * | *** | * | * |
| Apoa1 | – | – | *** | * | – | *** | * | *** | * | * |
| Serpina1 | – | – | *** | *** | – | *** | *** | *** | *** | *** |
| Alb | – | – | *** | ** | – | *** | ** | *** | ** | – |
The statistical significance of the differential expression as determined by one-way ANOVA and Tukey's post-hoc analysis of the various genes is shown (-No significant difference in expression; *P ≥ 0.05; **P ≥ 0.01; ***P ≥ 0.005).
Column 1 is uninfected MEFs versus those expressing Hnf1 + Hnf4; column 2 is uninfected MEFs versus those expressing Hnf1 + Hnf4 + Gata4; column 3 is uninfected MEFs versus those expressing Hnf1 + Hnf4 + Foxa2/3; column 4 is uninfected MEFs versus those expressing Hnf1 + Hnf4 + Gata4 + Foxa2/3; column 5 is MEFs expressing Hnf1 + Hnf4 versus those expressing Hnf1 + Hnf4 + Gata4; column 6 is MEFs expressing Hnf1 + Hnf4 versus those expressing Hnf1 + Hnf4 + Foxa2/3; column 7 is MEFs expressing Hnf1 + Hnf4 versus those expressing Hnf1 + Hnf4 + Gata4 + Foxa2/3; column 8 is MEFs expressing Hnf1 + Hnf4 + Gata4 versus those expressing Hnf1 + Hnf4 + Foxa2/3; column 9 is MEFs expressing Hnf1 + Hnf4 + Gata4 versus those expressing Hnf1 + Hnf4 + Gata4 + Foxa2/3; and column 10 is MEFs expressing Hnf1 + Hnf4 + Foxa2/3 versus those expressing Hnf1 + Hnf4 + Gata4 + Foxa2/3.