Recombinant Human P450 Enzyme | Incubation Time | [14C]Laromustine | C-7 Formed per Incubation | [14C]Laromustine Detected per Incubation | Percent Loss of substratea |
---|---|---|---|---|---|
min | μ M | pmol | pmol | % | |
Bactosome control | 10 | 25 | NC | 5380 | 17.9% |
Reductase control | NC | 5390 | 17.7% | ||
rCYP1A2 | NC | 5560 | 15.0% | ||
rCYP2B6 | 376 | 5270 | 19.5% | ||
rCYP2D6 | NC | 5470 | 16.5% | ||
Bactosome control | 100 | NC | 20100 | 14.7% | |
Reductase control | NC | 20400 | 13.6% | ||
rCYP1A2 | NC | 20300 | 13.9% | ||
rCYP2B6 | 513 | 20000 | 15.3% | ||
rCYP2D6 | NC | 20100 | 14.6% |
NC, not calculated (represents a zero or negative value beyond the AUC level).
↵a Percent loss of substrate was determined on the basis of the zero-minute microsomal sample.