TABLE 1

Reproducibility assessment for control samples

Half-life values of the control samples across nine 384-well plates based metabolic stability assays were measured by UPLC/HRMS. Half-life categories: <10 min: low; >10 <30 min: moderate; >30 min: high.

Compound Name	Molecular Formula Parent)/Exact Mass [M + H]⁺	Linearity Range	No. of Repeats	t_1/2 (Mean + S.D.)/Category
		nMs
Buspirone	C₂₁H₃₁N₅O₂/386.2550	1–1000	18	2.5 ± 0.45 min/low
Loperamide	C₂₉H₃₃ClN₂O₂/477.2303	2–1000	18	3.5 ± 0.59 min/low
Ketoconazole	C₂₆H₂₈Cl₂N₄O₄/531.1560	4–1000	12^{^a}	21 ± 3.6 min/moderate
Antipyrine	C₁₁H₁₂N₂O/189.1022	5–1000	18	>>30^{^b} min/high
Carbamazepine	C₁₅H₁₂N₂O/237.1022	2–1000	18	>>30^{^b} min/high

↵^a Initial three experiments did not include ketoconazole as a control.
↵^b Half-lives of antipyrine and carbamazepine ranged from 150 to 400 minutes; hence, no S.D. was depicted.