TABLE 1

Kinetic constants for P450 inactivation by BPA and BG

The catalytic activities of WT, F206A, and V367A were assessed using the EFC de-ethylation activity assay and the kinetic values were determined as described in the Materials and Methods. The data shown represent the mean values of two independent experiments performed in triplicate.

Inactivator	P450	K_I	k_inact	k_inact/K_I
		μM	min⁻¹	min^-1mM⁻¹
BPA	WT	2.7	0.20	74
	F206A	141	0.28	2
	V367A	44	0.26	6
BG	WT	11.6	0.15	13
	F206A	2.2	0.13	59
	V367A	5.7	0.10	18