TABLE 2

Kinetic constants for BG inhibition of EFC de-ethylation activity catalyzed by WT and the F206A mutant

The catalytic activities were determined using EFC concentrations from 1 μM to 100 μΜ with different BG concentrations as described in the Materials and Methods.

P450	BG	K_m^{^a}	k_obs^{^a}	K_i^{^b}
	μM	μM	min⁻¹	μM
WT	0	1.7	0.80	0.12
	0.2	4.2	0.60
	0.3	5.0	0.54
	0.5	14	0.50
	1	20	0.41
F206A	0	3.6	0.56	0.004
	0.003	4.6	0.50
	0.005	4.9	0.44
	0.01	7.3	0.39
	0.02	13	0.34

↵^a The data from Fig. 7A (WT) and Fig. 8A (F206A) were fitted to the Michaelis–Menten equation using GraphPad Prism software.
↵^b The data were obtained by global fitting to a mixed-model inhibition equation using GraphPad Prism software. The values are the mean of two separate experiments run in triplicate.