Activity | High-Affinity Activity | Low-Affinity Activity | |||||
---|---|---|---|---|---|---|---|
Km | Vmax | Vmax/Km | Km | Vmax | Vmax/Km | ΣVmax/Km | |
μM | pmol/min per mg protein | ml/min per g protein | μM | pmol/min per mg protein | ml/min per g protein | ||
(+)-M1 | 7.0 | 248 | 35 | 554 | 329 | 0.6 | 36 |
(−)-M1 | 9.8 | 92 | 9.3 | 117 | 13 | 0.11 | 9.4 |
(+)-M2 | 69 | 440 | 6.4 | 5442 | 843 | 0.15 | 6.6 |
(−)-M2 | 49 | 235 | 4.6 | 2494 | 318 | 0.12 | 4.7 |
The data points used for fitting were the average of two independent experiments performed in duplicate (data points shown in Fig. 4 with the curves of best fit). Fitted parameters included Km and Vmax, while intrinsic clearance (Vmax/Km) values were calculated. Data for (+)-M1 and (−)-M1 formation were best fit by a two-enzyme model. Kinetic parameters for high- and low-affinity activities, as well as the sum of the high and low intrinsic clearance values (ΣVmax/Km), are given.