Concentration-dependent uptake of LY2140023 into HeLa cells to estimate kinetic parameters
The concentration dependent transport of LY2140023 was evaluated from either 5 to 146 (experiment 1) or 6.4 to 149 μM (experiment 2) LY2140023. All concentrations were performed in triplicate. The estimated Km and Vmax values represent parameter ± S.E.M.
| Experiment | Km | Vmax |
|---|---|---|
| μM | pmol/min/mg | |
| Experiment 1 | 28.66 ± 5.01 | 238.3 ± 20.8 |
| Experiment 2 | 29.98 ± 5.29 | 572.8 ± 49.6 |