Comparison of efflux active P-gp with total P-gp mRNA in MDCKII-hMDR1-NKI and Caco-2 cells
| P-gp mRNA | Total P-gp Levels by LC-MS/MS | Ratio of P-gp mRNA (copies/ng mRNA) to P-gp Protein per Transwell | |
|---|---|---|---|
| Copies of P-gp mRNA/ng RNA | pmol P-gp per Cell Monolayer of a Transwella | ||
| MDCKII-hMDR1-NKI | 6748 ± 653 | 0.78 ± 0.26 | (9 ± 3) × 103 |
| Caco-2 | 918 ± 78 | 0.95 ± 0.27 | (1 ± 0.3) × 103 |
| Ratio MDCK/Caco-2 | 7.4 ± 0.9 | 0.8 ± 0.4 |
↵a pmol P-gp per single transwell of 12-transwell plates. Value is mean ± S.D. (n = 3).