Primers used in real-time quantitative PCR
| Gene | Direction | Sequence(5′→3′) | Product Length |
|---|---|---|---|
| bp | |||
| hENT1 | Forward | CAGAATGTGTCCTTGGTCACT | 512 |
| Reverse | ATGATAACAGCACAGGCTGTG | ||
| hENT2 | Forward | CCTCCGTCTGCTTCATCAACT | 470 |
| Reverse | CTGGAAGACAGTGAAGACTGA | ||
| hCNT1 | Forward | TGGAAGGTCTGGGACATGGAGAA | 612 |
| Reverse | ATGATGCTTTGAGCAGGCAA | ||
| hCNT2 | Forward | AAGAAGTAGAGCCTGAGGGAA | 386 |
| Reverse | AACCAAGGAGACTCCTGCAAA | ||
| hCNT3 | Forward | GAGAACGAGAACACATCAGGA | 438 |
| Reverse | CCAGAACCAATGGCTGTTTAG | ||
| hOCT3 | Forward | TCGCTCTGTTCAGGTCTGTG | 115 |
| Reverse | TGGATGCCAGGATACCAAAG | ||
| hOCTN1 | Forward | CGGAATATTGCCATAATGACC | 72 |
| Reverse | CAGAGCAAAGTAACCCACTGAG | ||
| hOCTN2 | Forward | GCAGCATCCTGTCTCCCTAC | 91 |
| Reverse | GCTGTCAGGATGGTCAGACTT | ||
| hOAT4 | Forward | CTGTGGAAAGTACCTCGCTCT | 120 |
| Reverse | CTTGAAGTCGCCCAACTCG | ||
| hBCRP | Forward | CCACTCCCACTGAGATTGAGA | 73 |
| Reverse | TGCGTTCCTAAATCCTACCC | ||
| hP-gp | Forward | GAAATTTAGAAGATCTGATGTCAAACA | 110 |
| Reverse | ACTGTAATAATAGGCATACCTGGTCA | ||
| hMRP2 | Forward | AGTGAATGACATCTTCACGTTTG | 63 |
| Reverse | CTTGCAAAGGAGATCAGCAA | ||
| hGADPH | Forward | GCACCGTCAAGGCTGAGAAC | 138 |
| Reverse | TGGTGAAGACGCCAGTGGA |