Apparent kinetic parameters of luteolin, chrysoeriol, and diosmetin glucuronidation obtained from RLS9
Calculations were based on curve fitting using the Michaelis–Menten equation, substrate inhibition, or autoactivation model, as described in Materials and Methods. Data are expressed as the mean ± S.D., n = 3.
| Metabolites | Km (μmol/L) | Vmax (nmol/mg/min) | CLint (mL/min/mg) | Ki (μmol/L) | |
|---|---|---|---|---|---|
| Luteolin | Lut-7-Ga | 5.5 ± 1.1 | 5.3 ± 0.5 | 1.0 ± 0.3 | 226 ± 100 |
| Lut-4′-Ga | 2.2 ± 0.5 | 0.9 ± 0.1 | 0.4 ± 0.1 | 221 ± 98 | |
| Lut-3′-Gb | 8.6 ± 1.2 | 6.0 ± 0.3 | 0.7 ± 0.1 | ||
| Chrysoeriol | Chr-7-Gb | 3.5 ± 0.3 | 3.9 ± 0.1 | 1.1 ± 0.1 | |
| Chr-4′-Gb | 0.3 ± 0.1 | 2.3 ± 0.1 | 8.2 ± 2.5 | ||
| Diosmetin | Dio-7-Gc | 6.2 ± 1.6 | 3.8 ± 0.5 | 0.6 ± 0.1 | |
| Dio-3′-Ga | 2.5 ± 0.4 | 6.1 ± 0.4 | 2.5 ± 0.2 | 425 ± 246 |