TABLE 2

Intra- and interday precision and accuracy for LC-MS/MS quantification of P450 probe drugs and corresponding metabolites in mouse plasma

Concentrations are given in nanomoles. Values represent means ± S.D. or percentages unless otherwise indicated.

AnalyteNominal 
ConcentrationIntra-assay (n = 3)Interassay (n = 9)
Measured 
ConcentrationRelevant
S.D.AccuracyMeasured 
ConcentrationRelevant
S.D.Accuracy
PHE7569.93 ± 18.7026.793.275.69 ± 10.8414.3101
400387.7 ± 20.85.3896.8394.9 ± 15.43.8998.6
40003580 ± 1143.1789.43707 ± 1684.5492.6
APAP1514.97 ± 1.8512.499.915.69 ± 1.237.84104
8081.57 ± 0.961.1810278.96 ± 2.383.0298.7
800699.7 ± 11.81.6987.4722.6 ± 36.55.0590.3
MDZ1515.40 ± 1.9512.710314.63 ± 1.9813.697.5
8073.77 ± 5.136.9592.277.96 ± 9.2911.997.4
800688.7 ± 66.39.6286.1721.3 ± 57.27.9390.2
1′-OH-MDZ7571.47 ± 3.504.9095.474.56 ± 6.188.2999.4
400382.3 ± 23.06.0295.6378.0 ± 14.43.8094.5
40003523 ± 1363.8688.13661 ± 2727.4291.6
DXM7564.67 ± 10.8016.783.066.46 ± 7.4711.287.5
80356.0 ± 14.74.1489.0371.8 ± 19.65.2792.9
8003767 ± 1864.9394.23741 ± 1614.2993.6
DXO1512.67 ± 2.6320.784.612.87 ± 2.1416.785.8
40067.90 ± 4.486.6084.972.92 ± 5.457.4891.1
4000700.0 ± 25.93.7187.5736.8 ± 39.45.3592.1
DIC7586.20 ± 6.777.8611590.33 ± 21.5623.9121
400468.3 ± 11.22.40117421.8 ± 48.611.5106
40003647 ± 1233.3891.14148 ± 60414.6104
4′-OH-DIC1515.70 ± 2.4815.810517.61 ± 3.0217.2118
8086.87 ± 15.7618.210981.39 ± 12.6515.6102
800826.3 ± 20.22.45103876.6 ± 102.811.7110
CLZ7587.60 ± 8.8810.111792.79 ± 16.5017.8124
400429.3 ± 51.612.0107413.4 ± 52.212.6103
40003716 ± 3118.3692.94290 ± 70016.3107
6′-OH-CLZ1516.33 ± 4.7128.810916.64 ± 3.6021.6111
8084.17 ± 14.116.810581.91 ± 34.7812.8116
800678.3 ± 157.823.384.8840.1 ± 194.523.2105