TABLE 3

DME activities of cryopreserved human enterocytes

Enterocytes from 4 male (HE3005, HE3007, HE3008, HE3010) and 4 females (HE3006, HE3009, HE3011, HE3013) were pooled and used in the study. The DME pathways evaluated were as follows: CYP1A2 (phenacetin hydroxylation), CYP2A6 (coumarin 7-hydroxylation), CYP2B6 (bupropion hydroxylation), CYP2C8 (paclitaxel 6α-hydroxylation), CYP2C9 (diclofenac 4-hydroxylation), CYP2C19 (S-mephenytoin 4-hydroxylation), CYP2D6 (dextromethorphan hydroxylation), CYP2E1 (chlorzoxazone 6-hydroxylation), CYP3A4 [midazolam 1′-hydroxylation (CYP3A4M) and testosterone 6β-hydroxylation (CYP3A4T)], CYP2J2 (astemizole O-demethylation), UGT (7-hydroxycoumarin glucuronidation), SULT (7-hydroxycoumarin sulfation), and CES2 (irinotecan hydrolysis) activities. Results are mean and S.D. of triplicate determinations. Activities for CYP1A2, CYP2A6, CYP2B6, and CYP2D6 were below quantifiable levels (BQL).