TABLE 4

K_p,uu,ss and f_T,cell,ss in human hepatocytes

Values are shown as the mean ± S.D. (n = 3).

Drug	Lot	C/M Ratio^{^c}		K_p,uu,ss^{^d}	f_T,cell,ss^{^d}
		37°C	On ice
Diazepam	Hu8075^{^a}	216 ± 20	181 ± 38	1.19 ± 0.51	0.00553 ± 0.00115
	TFF^{^b}	124 ± 53	303 ± 22	0.409 ± 0.247	0.00330 ± 0.00024
Pitavastatin	Hu8075^{^a}	471 ± 88	35.2 ± 2.6	13.4 ± 2.7	0.0284 ± 0.0021
	TFF^{^b}	150 ± 2.6	21.8 ± 3.2	6.92 ± 1.02	0.0460 ± 0.0068
Rosuvastatin	Hu8075^{^a}	52.2 ± 8.6	4.51 ± 0.41	11.6 ± 2.2	0.222 ± 0.020
	TFF^{^b}	27.2 ± 2.5	4.28 ± 0.53	6.36 ± 1.06	0.234 ± 0.029
Pravastatin	Hu8075^{^a}	3.73 ± 0.73	1.84 ± 0.12	2.03 ± 0.54	0.545 ± 0.035
	TFF^{^b}	2.65 ± 1.29	2.07 ± 0.64	1.28 ± 0.89	0.484 ± 0.151

↵^a Isolated cryopreserved human hepatocytes (Lot Hu8075) were incubated with diazepam (0.2 µM), pitavastatin (0.1 µM), rosuvastatin (0.1 µM), and pravastatin (0.2 µM).
↵^b Pooled cryopreserved human hepatocytes from 20 mixed-sex donors (Lot TFF) were incubated with diazepam (1 µM), pitavastatin (0.5 µM), rosuvastatin (0.5 µM), and pravastatin (1 µM).
↵^c C/M ratios at 37°C and on ice were calculated using the uptake data at 30 minutes and 60 minutes, respectively (Fig. 2).
↵^d The K_p,uu,ss and f_T,cell,ss were calculated from C/M ratios using eqs. 2 and 3, respectively.