Primer sequences used for the cloning of mouse Cyp2c65, Cyp2c67, Cyp2c68, and Cyp2c69 cDNAs
Corresponding positions are relative to the ATG initiating codon designated as +1.
| cDNA and Direction | Primer Sequence 5′ to 3′ | Corresponding Position |
|---|---|---|
| Cyp2c65 | ||
| Forward | GCAAACAATGGTTCTAGGTGTGTT | −6 to +17 |
| Reverse | CCATCAGATAAGAACAGGGCTGA | +1807 to +1829 |
| Cyp2c67 | ||
| Forward | GATCCATTTGTAGTCTTGGTGCTTTGTC | +4 to +31 |
| Reverse | ATAGCAGAATCTTTCCTCATCTTCACTT | +1491 to +1518 |
| Cyp2c68 | ||
| Forward | ATCATACCAGTGTCAGTAAGAAGGG | −29 to -5 |
| Reverse | ACTGTTAGCCTTGGGCAATTAGATA | +1605 to +1629 |
| Cyp2c69 | ||
| Forward | GAATGCCTGATCATAACAGTGTCAG | −38 to -14 |
| Reverse | ATAACAGAGAACTTTCAGCCTTGGG | +1611 to +1635 |