Mean percentage of the [14C]-radioactivity of the most abundant metabolites in AUC pooled human plasma (0–24 h) and their corresponding percentage of the DRM in pooled urine (0–24 h) and feces (0–72 h)
| Parent and Metabolites | Matrix | ||||
|---|---|---|---|---|---|
| ID | Accurate MH+ | ∆ppm | Plasma | Urine | Feces |
| % of [14C] AUC | % of DRM | % of DRM | |||
| MMB | 415.1876 | −0.2 | 17.3 | 0.6 | 12.6 |
| M21 | 429.1663 | −1.6 | 64.2 | 11.5 | 12.7 |
| M8 | 447.1768 | −1.3 | 5.8 | 0.7 | 2.5 |
| M19 | 377.1604 | −1.1 | 5.2 | ND | 7.1a |
| M5 | 461.1564 | −0.9 | 2.7 | 2.3 | 3.8 |
| M28 | 391.1398 | −0.8 | 2.5 | 1.0 | ND |
| M20 | 387.1565 | +0.3 | 2.3 | ND | 1.7 |
| M14b | 390.1554 | −1.8 | ND | 1.8 | 21.4 |
| M16c | 389.1713 | −2.1 | ND | 1.6 | 1.5 |
| M1d | 591.2188 | −1.7 | ND | 1.5e | ND |
MH+, protonated molecular ion; ND, not detected; ∆ppm, mass accuracy expressed in parts per million.
↵a M19 coelute with M33 (m/z = 431.1469, oxidative metabolite of MMB) in human feces.
↵b M14 was listed because it was a major metabolite in human feces.
↵c M16 was listed because it represented >10% of DRM in bile duct-cannulated dog urine (Supplemental Table 2).
↵d M1 was listed because it represented a phase II metabolism pathway in human.
↵e M1, a direct glucuronide of MMB, coeluted with the oxidation plus glucuronidation of M21 (m/z = 621.1921) in human urine.