TABLE 1
Relationship between BSA concentration (unbound fraction) and PSu,inf of ANS
| BSA (%) | 0 | 0.016 | 0.094 | 0.25 | 0.51 |
|---|---|---|---|---|---|
| Unbound fraction | 1 | 0.486 | 0.126 | 0.0487 | 0.0231 |
 a (μl/min per milligram protein) | 60.3 ± 5.0 | 69.1 ± 12.9 | 84.0 ± 4.6 | 113 ± 15 | 130 ± 10 |
 b (μl/min per milligram protein) | 62.7 ± 1.4 | 83.9 ± 0.4 | 120 ± 14 | 171 ± 19 | 159 ± 33 |
↵a Data shown in Fig. 5. ANS hepatic uptakes were determined as ANS and BSA concentrations were varied, and the unbound concentration of ANS was kept constant at approximately 2.4 μM.
↵b Data shown in Fig. 6. ANS uptakes were determined with varying BSA concentrations and keeping ANS concentration constant at 20 μM.